Environmental & Metabolic Health Profile

TOXDetect Profile® + MycoTOX Profile® + Organic Acids Test (OAT)

Comprehensive Urine Testing for Environmental Toxicants, Mycotoxins & Metabolic Health

One patient. One collection kit. Three tests. 114 markers.

The Environmental & Metabolic Health Profile combines three comprehensive urine-based laboratory tests to provide a broader picture of selected environmental toxicant exposure, mycotoxin exposure, metabolic pathways, nutritional markers and microbial activity.

This package includes:

• TOXDetect Profile® – 27 environmental toxicant markers

• MycoTOX Profile® – 11 mycotoxin markers

• Organic Acids Test (OAT) – 76 organic acid markers

114 Markers in One Comprehensive Package

All three tests use the same type of urine collection kit, so only one kit is required for one patient.

A total of 30 mL of urine is required:

• 10 mL for TOXDetect Profile®

• 10 mL for MycoTOX Profile®

• 10 mL for Organic Acids Test (OAT)

IMPORTANT: The urine sample must be frozen after collection and must arrive at the laboratory frozen.

Please follow all collection, freezing, storage and shipping instructions supplied with your testing kit.

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What Does This Package Assess?

The three tests look at different areas of health and exposure.

TOXDetect Profile®

Screens for selected metabolites associated with environmental toxicants, including:

• Phthalates

• Bisphenols

• Volatile Organic Compounds (VOCs)

• Parabens

• Pesticides

• Glyphosate

• Additional environmental chemicals and industrial compounds

MycoTOX Profile®

Assesses 11 selected mycotoxins associated with mould and fungal exposure.

Organic Acids Test (OAT)

Measures 76 organic acids associated with:

• Energy metabolism

• Mitochondrial pathways

• Nutritional status

• Vitamin-related pathways

• Neurotransmitter metabolism

• Microbial metabolism

• Yeast and fungal metabolism

• Fatty-acid and ketone metabolism

• Amino-acid metabolism

• Oxalate metabolism

• Oxidative stress and related metabolic pathways

Together, these tests provide information across 114 laboratory markers.

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1. TOXDetect Profile®

Environmental Toxicant Exposure Testing

TOXDetect Profile® is an advanced urine-based laboratory test designed to measure key metabolites associated with selected environmental toxicants.

Environmental toxicants are substances present in the environment that may enter the body through ingestion, inhalation or absorption.

Potential sources include:

• Plastics and consumer products

• Household products

• Pesticides

• Agricultural sources

• Industrial environments

• Building materials

• Tobacco smoke

• Vehicle emissions

• Solvents and other chemicals

• Other environmental sources

Environmental toxicant exposure has been associated with a range of biological effects, including oxidative stress, hormone disruption, immune effects and neurological concerns.

The TOXDetect Profile® can provide additional information about selected environmental chemical exposures that may be considered alongside an individual’s symptoms, medical history, lifestyle and environmental history.

What Does TOXDetect Screen For?

Phthalates

Phthalates are chemicals commonly used as plasticisers to make plastics more flexible and durable.

The test measures:

• Monoethyl phthalate (MEP)

• Monobutyl phthalate (MBP)

• Mono-2ethylhexyl phthalate (MEHP)

• Mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP)

• Monoisobutyl phthalate (MiBP)

Volatile Organic Compounds (VOCs)

Markers include:

• 2-3-4 Methylhippuric Acid (2-3-4-MHA)

• Phenylglyoxylic Acid (PGO)

• N-Acetyl Phenyl Cysteine (NAP)

• N-Acetyl (2-Cyanoethyl) Cysteine (NACE)

• N-Acetyl (Propyl) Cysteine (NAPR)

• N-Acetyl (3,4-Dihydroxybutyl) Cysteine (NADB)

• 2-Hydroxyethyl Mercapturic Acid (HEMA)

These metabolites are associated with exposure to compounds including xylene, styrene/ethylbenzene, benzene, acrylonitrile, 1-bromopropane, 1,3-butadiene, ethylene oxide and vinyl chloride.

Pesticides

The test includes:

• 3-Phenoxybenzoic Acid (3-PBA)

• Diethyl Phosphate (DEP)

• 2,4-Dichlorophenoxyacetic Acid (2,4-D)

Additional Environmental Chemicals

The test also includes:

• Diphenyl Phosphate (DPP)

• N-Acetyl (Carbomethyl) Cysteine (NAE)

• Perchlorate (PERC)

• Bisphenol S (BPS)

• Bisphenol A (BPA)

• Methylparaben (MeP)

• Ethylparaben (EtP)

• Propylparaben (PrP)

• Butylparaben (BuP)

• Atrazine Mercapturate (AM)

• Oxybenzone (OBZ)

• Glyphosate

TOXDetect Profile® Includes 27 Markers

The results provide laboratory measurements that can help your healthcare professional consider potential environmental exposure alongside your wider clinical picture.

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2. MycoTOX Profile®

Urine Mycotoxin Testing

Mycotoxins are metabolites produced by certain moulds and fungi.

Mould can be present in:

• Damp or water-damaged buildings

• Homes and workplaces

• Vehicles

• Building materials

• Food

• Other environmental sources

Exposure can occur through different routes, including inhalation and ingestion.

The MycoTOX Profile® is a comprehensive urine-based laboratory assessment designed to measure selected mycotoxins and provide information about potential exposure.

What Does MycoTOX Measure?

The MycoTOX Profile® measures 11 different mycotoxins associated with multiple mould species.

Mycotoxin Markers

• Aflatoxin M1 (AFM1)

• Ochratoxin A

• Gliotoxin (GTX)

• Sterigmatocystin (STC)

• Roridin E (ROE)

• Verrucarin A (VRA)

• Enniatin B (ENB)

• Zearalenone

• Chaetoglobosin A (CHA)

• Mycophenolic Acid (MPA)

• Citrinin / Dihydrocitrinone (DHC)

The MycoTOX Profile® can be considered alongside environmental history, symptoms and other relevant investigations when assessing possible mould or mycotoxin exposure.

MycoTOX Profile® Includes 11 Mycotoxin Markers

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3. Organic Acids Test (OAT)

Comprehensive Metabolic, Nutritional & Microbial Assessment

The Organic Acids Test (OAT) is a urine-based laboratory test measuring 76 organic acids produced through normal metabolism and microbial activity.

Organic acids are metabolic by-products produced by the body and microorganisms such as bacteria and fungi.

They are generated through biological pathways involved in:

• Energy production

• Nutrient metabolism

• Neurotransmitter metabolism

• Microbial activity

• Fatty-acid metabolism

• Amino-acid metabolism

• Other physiological processes

The OAT provides a broad overview of selected metabolic pathways and can be used alongside clinical history and other investigations.

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OAT Markers

Microbial, Yeast & Bacterial Markers

• Citramalic Acid

• 5-Hydroxymethyl-2-furoic Acid

• 3-Oxoglutaric Acid

• Furan-2,5-dicarboxylic Acid

• Furancarbonylglycine

• Tartaric Acid

• Arabinose

• HPHPA

• 4-Cresol

• DHPPA

Gut & Microbial Metabolism

• Carboxycitric Acid

• Tricarballylic Acid

• 2-Hydroxyphenylacetic Acid

• 4-Hydroxyphenylacetic Acid

• 4-Hydroxybenzoic Acid

• 4-Hydroxyhippuric Acid

• Hippuric Acid

• 3-Indoleacetic Acid

Mitochondrial & Energy Metabolism

• Succinic Acid

• Fumaric Acid

• Malic Acid

• 2-Oxoglutaric Acid

• Aconitic Acid

• Citric Acid

• Lactic Acid

• Pyruvic Acid

• 2-Hydroxybutyric Acid

Fatty-Acid & Ketone Metabolism

• 3-Hydroxybutyric Acid

• Acetoacetic Acid

• 4-Hydroxybutyric Acid

• Ethylmalonic Acid

• Methylsuccinic Acid

• Adipic Acid

• Suberic Acid

• Sebacic Acid

Neurotransmitter Metabolism

• Homovanillic Acid (HVA)

• Vanillylmandelic Acid (VMA)

• HVA/VMA Ratio

• 5-Hydroxyindoleacetic Acid (5-HIAA)

• Quinolinic Acid

• Kynurenic Acid

• HVA/DOPAC Ratio

• Dihydroxyphenylacetic Acid (DOPAC)

Nutritional & Vitamin-Related Markers

• Methylmalonic Acid – Vitamin B12

• Pyridoxic Acid – Vitamin B6

• Pantothenic Acid – Vitamin B5

• Glutaric Acid – Vitamin B2/Riboflavin

• Ascorbic Acid – Vitamin C

• 3-OH-3-Methylglutaric Acid – CoQ10

• Methylcitric Acid – Biotin

• N-Acetylcysteine (NAC)

• Pyroglutamic Acid

Oxalate Metabolism

• Oxalic Acid

• Glyceric Acid

• Glycolic Acid

Amino-Acid & Related Metabolism

• Orotic Acid

• 2-Hydroxyhippuric Acid

• 2-Hydroxyisovaleric Acid

• 2-Oxoisovaleric Acid

• 3-Methyl-2-oxovaleric Acid

• 2-Hydroxyisocaproic Acid

• 2-Oxoisocaproic Acid

• 2-Oxo-4-methiolbutyric Acid

• Mandelic Acid

• Phenyllactic Acid

• Phenylpyruvic Acid

• Homogentisic Acid

• 4-Hydroxyphenyllactic Acid

• N-Acetylaspartic Acid

• Malonic Acid

• 3-Methylglutaric Acid

• 3-Hydroxyglutaric Acid

• 3-Methylglutaconic Acid

• Uracil

• Thymine

• Phosphoric Acid

Organic Acids Test Includes 76 Markers

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What Can the OAT Assess?

The OAT examines markers associated with several important metabolic pathways.

Mitochondrial & Energy Production

Markers such as succinic acid, fumaric acid, malic acid, 2-oxoglutaric acid, aconitic acid and citric acid are involved in pathways associated with cellular energy production.

Glycolytic & Energy Metabolism

Lactic acid and pyruvic acid are associated with glucose metabolism and cellular energy production.

Nutritional Status

The OAT includes markers associated with:

• Vitamin B12

• Vitamin B6

• Vitamin B5

• Vitamin B2/Riboflavin

• Vitamin C

• CoQ10

• Biotin

• N-acetylcysteine (NAC)

Neurotransmitter Metabolism

Markers include:

• HVA

• VMA

• HVA/VMA ratio

• DOPAC

• 5-HIAA

• Quinolinic Acid

• Kynurenic Acid

These metabolites are involved in neurotransmitter-related pathways.

Yeast, Fungal & Bacterial Metabolism

The OAT includes markers associated with microbial metabolism, including:

• Arabinose

• Citramalic Acid

• Tartaric Acid

• HPHPA

• 4-Cresol

• DHPPA

These findings can be considered alongside symptoms, medical history and other investigations.

Detoxification & Oxidative Stress

The OAT includes metabolites associated with pathways involved in:

• Cellular detoxification

• Antioxidant metabolism

• Oxidative stress

• Glutathione-related metabolism

Oxalate Metabolism

Markers include:

• Oxalic Acid

• Glyceric Acid

• Glycolic Acid

These provide information about aspects of oxalate metabolism.

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Why Combine These Three Tests?

The three tests examine different biological areas.

TOXDetect Profile®

Environmental toxicants

Provides information about selected metabolites associated with environmental chemicals, pesticides, plasticisers, VOCs, parabens, bisphenols and other compounds.

MycoTOX Profile®

Mycotoxins

Provides information about 11 selected mycotoxins associated with mould and fungal exposure.

Organic Acids Test

Metabolic, nutritional & microbial pathways

Provides information across 76 organic acid markers associated with energy metabolism, nutritional status, neurotransmitter metabolism and microbial activity.

By combining the three, you receive a broader set of laboratory data that can be considered alongside your individual health history and other investigations.

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Who May Consider This Testing Package?

This package may be considered as part of a broader investigation for individuals with persistent, unexplained or complex symptoms, or those with concerns about environmental exposure.

This may include individuals experiencing:

• Persistent fatigue or low energy

• Brain fog or cognitive symptoms

• Chronic sinus or respiratory concerns

• Digestive or gut-related symptoms

• Chemical sensitivities

• Hormonal or metabolic concerns

• Neurological symptoms

• Recurrent health concerns

• Concerns about mould exposure

• A history of living or working in a damp or water-damaged environment

• Concerns about environmental chemical exposure

• Nutritional or metabolic concerns

• Complex or multi-system health concerns

The OAT may also be considered as part of broader investigations involving ADHD-related symptoms, autism-related concerns, chronic fatigue, mould or environmental health concerns, histamine or MCAS-related symptoms and persistent gut-related symptoms.

Important: These symptoms and conditions can have many possible causes. The tests should not be used alone to diagnose or explain a medical condition.

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Your Testing Journey

Step 1 – Order Your Test

Once your order has been placed, your collection kit and preparation instructions will be provided.

Because all three tests use the same kit type, only one kit is required for one patient.

Step 2 – Prepare for Testing

Please carefully follow the preparation instructions supplied with your kit.

Certain foods, supplements, medications and treatments may influence some results.

Some items may need to be avoided for up to three days before collection.

Do not discontinue prescribed medication or supplements without first consulting your healthcare professional.

Step 3 – Collect Your Urine

The complete package requires 30 mL of urine in total:

• 10 mL for TOXDetect Profile®

• 10 mL for MycoTOX Profile®

• 10 mL for OAT

For the OAT, the recommended sample is 10 mL of first-morning urine collected before food or drink.

Follow the specific collection instructions supplied with your kit.

Step 4 – FREEZE YOUR SAMPLE

IMPORTANT: THE SAMPLE MUST BE FROZEN

After collection, the urine sample must be frozen according to the instructions provided with your kit.

The laboratory must receive the sample frozen.

Please keep the sample frozen until it is prepared for shipping.

Do not send an unfrozen sample.

Step 5 – Return Your Sample

Our fees include postage and packaging to and from the client and laboratory.

A printer is required to print the laboratory return label.

Step 6 – Sample Dispatch

Because the laboratory requires the sample to arrive frozen, Human Defences sends samples to Mosaic Labs on Mondays and Tuesdays.

We can receive samples from clients on other days, but onward dispatch to Mosaic Labs takes place on Monday and Tuesday.

Please plan your collection and shipping accordingly.

Step 7 – Laboratory Analysis

Your sample will be sent to the laboratory for analysis.

The three tests are analysed separately.

Step 8 – Receive Your Results

Your laboratory reports will be sent to you by email.

Because the three tests are processed separately, you may receive the reports at different times.

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Important Preparation Information

Food Restrictions for the OAT

For 48 hours before OAT sample collection, avoid:

• Apples

• Grapes, including raisins

• Pears

• Cranberries and their juices

Supplements to Avoid Before OAT

For 48 hours before collection, avoid:

• Arabinogalactan

• Echinacea

• Reishi mushrooms

• Ribose

Additional foods, supplements or medications may need to be avoided for up to three days before testing.

Follow the complete preparation instructions provided with your kit.

Important

Do not stop prescribed medications or supplements without first discussing this with your healthcare professional.

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Antibiotics & Antimicrobial Treatments

For the OAT, please wait at least 48 hours after completing a course of antibiotics before collecting your sample, unless your healthcare professional advises otherwise.

Antimicrobial or antifungal treatments may also influence certain results.

If you are currently taking medication or undergoing treatment, please discuss preparation requirements with your healthcare professional.

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Sample Requirements

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Turnaround Times

Turnaround times are measured from the point at which the laboratory receives the specimen.

TOXDetect Profile®

Approximately 5–10 business days after the laboratory receives the specimen.

Organic Acids Test (OAT)

Approximately 1–2 weeks after the laboratory receives the sample.

MycoTOX Profile®

Please refer to the current turnaround information supplied with the MycoTOX testing kit.

Please note: Shipping time is additional to laboratory processing time.

As the tests are processed separately, reports may be issued at different times.

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Postage & Shipping

The package price includes postage and packaging to and from the client and the laboratory.

A printer will be required to print the laboratory return label.

Because the laboratory must receive the sample frozen, Human Defences sends samples to Mosaic Labs on:

Monday & Tuesday

Samples can be received by Human Defences on other days, but they will be forwarded to Mosaic Labs on the next appropriate Monday or Tuesday dispatch.

Please follow all freezing, packaging and shipping instructions supplied with your kit.

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Your Results

Your completed laboratory reports will be sent to you by email.

The reports provide laboratory measurements for the individual tests included in the package.

Results should be considered in the context of:

• Your medical history

• Current symptoms

• Diet

• Nutritional status

• Medication and supplement use

• Environmental history

• Occupational exposure

• Lifestyle

• Other laboratory investigations

Results outside a laboratory reference range can have a number of possible explanations and should not automatically be interpreted as evidence of disease or toxicity.

Similarly, detection of a biomarker does not necessarily establish the source, timing or clinical significance of an exposure.

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What Happens After Testing?

Depending on the findings and your individual circumstances, an appropriately qualified healthcare professional may consider factors such as:

• Environmental exposure history

• Damp or mould-affected environments

• Dietary patterns

• Nutritional status

• Supplementation

• Medication

• Microbial activity

• Energy metabolism

• Environmental or occupational exposures

• Further investigations

• Appropriate dietary or lifestyle changes

Where appropriate, your healthcare professional may use the laboratory findings as one part of a broader assessment.

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Professional Interpretation & Clinical Guidance

Please be aware:

Human Defences is unable to provide interpretation support or clinical guidance based on your test results.

For the best possible advice, it is important to consult an appropriately qualified healthcare professional who can consider your full medical history, symptoms, medications, nutritional status, environmental history and other relevant test results when reviewing your reports.

Your healthcare professional can help determine whether further investigation or any appropriate intervention is required.

If you require assistance finding an appropriate healthcare professional to discuss your results, please let us know.

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Frequently Asked Questions

Is this package for one patient?

Yes.

This package is designed for one patient and includes three tests:

• TOXDetect Profile®

• MycoTOX Profile®

• Organic Acids Test (OAT)

Do I need three collection kits?

No.

All three tests use the same type of collection kit, so one collection kit is required for one patient.

How much urine is required?

You need 30 mL of urine in total:

• 10 mL for TOXDetect Profile®

• 10 mL for MycoTOX Profile®

• 10 mL for OAT

Does the urine sample need to be frozen?

Yes.

The urine sample must be frozen after collection and must arrive at the laboratory frozen.

This is an important laboratory requirement.

Please follow the freezing and storage instructions supplied with your kit.

When does Human Defences send samples to Mosaic Labs?

Human Defences sends samples to Mosaic Labs on Mondays and Tuesdays because the laboratory requires samples to arrive frozen.

We can receive samples on other days, but onward dispatch takes place on Monday or Tuesday.

Can I send my sample to Human Defences on another day?

Yes.

We can receive your sample on other days, but samples will only be forwarded to Mosaic Labs on the appropriate Monday or Tuesday dispatch.

Do I need a printer?

Yes.

A printer is required to print the laboratory return label.

Is postage included?

Yes.

Our fees include postage and packaging to and from the client and laboratory.

Is this a blood test?

No.

All three tests use urine samples.

What should I avoid before the OAT?

For 48 hours before collection, avoid:

• Apples

• Grapes and raisins

• Pears

• Cranberries and their juices

• Arabinogalactan

• Echinacea

• Reishi mushrooms

• Ribose

Additional preparation requirements may apply.

Can I stop my medication before testing?

No medication should be stopped without first discussing this with your healthcare professional.

How long after antibiotics should I wait?

For the OAT, wait at least 48 hours after completing a course of antibiotics before collecting your sample, unless your healthcare professional advises otherwise.

Will all three reports arrive together?

Not necessarily.

The three tests are processed separately, so you may receive the reports at different times.

How long do the results take?

Approximate laboratory processing times are:

• TOXDetect: 5–10 business days after laboratory receipt

• OAT: 1–2 weeks after laboratory receipt

• MycoTOX: according to the current laboratory turnaround time

Shipping time is additional.

Can these tests diagnose a medical condition?

No.

These tests provide laboratory measurements of selected biomarkers. They should not be used on their own to diagnose a medical condition or establish that a particular exposure caused a specific symptom or illness.

Who should interpret my results?

Your results should be discussed with an appropriately qualified healthcare professional who can consider your complete medical history and individual circumstances.

Human Defences does not provide clinical interpretation or medical guidance.

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At a Glance

Environmental & Metabolic Health Profile

Three Comprehensive Urine Tests

TOXDetect Profile®
27 environmental toxicant markers

MycoTOX Profile®
11 mycotoxin markers

Organic Acids Test (OAT)
76 organic acid markers

Total: 114 Markers

One patient

One collection kit

30 mL total urine

Frozen sample required

Laboratory reports delivered by email

Postage included

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Take a Broader Look at Environmental & Metabolic Health

The Environmental & Metabolic Health Profile brings together three complementary laboratory assessments in one convenient testing package.

By combining TOXDetect Profile®, MycoTOX Profile® and the Organic Acids Test, you can obtain a broader set of laboratory data covering selected environmental toxicants, mycotoxins and metabolic, nutritional and microbial markers.

All three tests are completed using the same type of urine collection kit for one patient, requiring a total of 30 mL of urine.

Important: Remember to Freeze Your Sample

The urine sample must be frozen after collection and must arrive at the laboratory frozen.

Human Defences sends samples to Mosaic Labs on Mondays and Tuesdays.

Please carefully follow all preparation, collection, freezing, storage and shipping instructions supplied with your kit.